Alcoholic xylose fermentation at high temperatures by the thermotolerant yeast hansenula polymorpha
AssigneeArcher Daniels Midland Company
InventorsCharles A. Abbas, Andriy A. Sibirny, Andriy Y. Voronovsky, Olena P. Ishchuk
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Abstract
Methods and compositions for the production of ethanol from lignocellulosic starting materials are provided herein. Embodiments provide yeast cells of the genus H. polymorpha with one or more modifications, including, for example, an inactive acid trehalase gene, overexpression of xylulokinase, and/or overexpression of heat-shock protein 104.
Claims20
An isolated ethanol-producing yeast of the species Hansenula polymorpha, wherein the yeast comprises at least one modification selected from the group consisting of:
- (a) an inactive ATH1 (acid trehalase) gene;
- (b) overexpression of the H. polymorpha protein xylulokinase when compared to a parent strain;
- and
- (c) overexpression of the H. polymorpha protein heat-shock protein 104 when compared to a parent strain.
A method of producing ethanol comprising:
- a) cultivating a yeast of claim 8 in a medium containing D-xylose as a carbon source, allowing ethanol to accumulate;
- and
- b) collecting ethanol from the medium, wherein said H. polymorpha yeast produces ethanol at levels higher than an H. polymorpha yeast having an active ATH1 gene.
The yeast of claim 12, wherein said non-native promoter is selected from the group consisting of the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter (GenBank #AY550078).
A method of producing ethanol comprising:
- a) cultivating the yeast of claim 14 in a medium containing D-xylose as a carbon source, allowing ethanol to accumulate;
- b) collecting ethanol from the medium, wherein said H. polymorpha yeast produces ethanol at levels higher than an H. polymorpha yeast that does not overexpress an H. polymorpha xylulokinase protein.
The method of claim 17, wherein said non-native promoter is selected from the group consisting of the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter (GenBank #AY550078).
A method of producing ethanol comprising:
- a) cultivating the yeast of claim 19 in a medium containing D-xylose as a carbon source, allowing ethanol to accumulate;
- and
- b) collecting ethanol from the medium, wherein said H. polymorpha yeast produces ethanol at levels higher than an H. polymorpha yeast that does not overexpress an H. polymorpha heat-shock 104 protein.
A strain of H. polymorpha deposited as NRRL Y-50061.
Dependencies are inferred from the claim text.