Ketoreductase polypeptides and uses thereof
AssigneeCodexis, Inc.
InventorsJACK LIANG, BIRTHE BORUP, VESNA MITCHELL, EMILY MUNDORFF, JAMES LALONDE, GJALT W. HUISMAN
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Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Claims80
A ketoreductase polypeptide capable of converting the substrate 2-[3-[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-oxopropyl]benzoic acid methyl ester to the product (S,E)-methyl 2-(3-(3-(2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-hydroxypropyl)benzoate at a rate that is at least 1.5 times the rate of the polypeptide of SEQ ID NO:4.
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Dependencies are inferred from the claim text.