METHOD FOR AMPLIFYING A FLAVIVIRUS cDNA IN A PROKARYOTIC CELL

Abstract

The invention relates to a method for amplifying a functional flavivirus cDNA in a prokaryotic cell, such as E. coli. The method involves a modified flavivirus cDNA that has one or more silent mutations in one or more prokaryotic promoter regions within a flavivirus cDNA. The silent mutation decreases or abolishes the promoter activity from the prokaryotic promoter region without resulting in a change to the amino acid sequence encoded by the modified flavivirus cDNA as compared to that encoded by the flavivirus cDNA. The invention also relates to the functional flavivirus cDNA generated by the method, its complement, and its RNA transcript. The invention further relates to vectors, host cells and flavivirus related to the functional flavivirus cDNA.

Dependencies are inferred from the claim text.