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US 2009/0155851 A1Published application
Novel process for preparation of chondroitin fraction
AssigneeSeikagaku Corporation
InventorsNobuo Sugiura, Koji Kimata
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AssigneeSeikagaku Corporation
InventorsNobuo Sugiura, Koji Kimata
Estimating the term…
Provided are a method for producing a fraction containing more than 50% of CH represented by the general formula (1), which comprises at least the step of allowing a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM to coexist, and performing a reaction thereof under conditions of 20 to 40° C. and pH 6 to 8 for 0.5 minutes to 4 hours, and a method for producing a fraction containing more than 50% of CH represented by the general formula (2), which comprises at least the step of performing the reaction under same conditions for 10 hours or longer, which enable industrial scale production of a CH fraction of a controlled even number saccharide and odd number saccharide content ratio by a simple procedure at a low cost. (GlcA-GalNAc)n (1) GalNAc-(GlcA-GalNAc)n (2) (In the formula, GlcA represents a glucuronic acid residue, GalNAc represents a N-acetylgalactosamine residue, - represents a glycosidic bond, and n represents an arbitrary integer.)
A method for producing a fraction comprising more than 50% of chondroitin represented by the general formula (1), which comprises the step of reacting a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM under conditions of 20 to 40° C. and pH 6 to 8 for 0.5 minutes to 4 hours,
A method for producing a fraction comprising chondroitin which comprises the step of reacting a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM under predetermined reaction conditions.
A method for producing a fraction comprising more than 50% of chondroitin represented by the general formula (2), which comprises the step of reacting a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 5 to 100 mM under conditions of 20 to 40° C. and pH 6 to 8 for 10 hours or longer.
14-16. (canceled)
A method according to claim 28, wherein the content ratio of ((1):(2)) is 45:55 to 55:45, the Mn2+ is present at a final concentration of 0.02 to 2 mM and reaction time is 5 hours or longer,
A method for producing a fraction comprising chondroitins represented by the general formulas (1) and (2) at a desired content ratio, which comprises the step of reacting a glucuronic acid donor and/or an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coil K4 strain, and Mn2+ under conditions of 20 to 40° C. and pH 6 to 8,
Dependencies are inferred from the claim text.