Generation of specific adhesion in gram-negative bacteria by means of anchoring immunoglobulin single domains on their surface with autotransporters
AssigneeConsejo Superior de Investigaciones Cientificas
InventorsEsteban Veiga Chacon, Victor De Lorenzo Prieto, Luis Angel Fernandez Herrero
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Abstract
The present invention refers to an expression vector for gram-negative bacteria allowing for the production of hybrid proteins between single domain recombinant antibodies and a transporter domain of an autotransporter, as well as to its anchoring and expression on the external surface of a bacterial membrane, and to a method of specific bacterial adhesion to a surface containing a specific antigen.
Claims34
A gene construction comprising:
- a) a first nucleic acid sequence containing the nucleotide sequence encoding a signal peptide involved in the passage of proteins across the cell membrane;
- b) a second nucleic acid sequence containing the nucleotide sequence encoding a single-domain recombinant antibody;
- and
- c) a third nucleic sequence containing the nucleotide sequence encoding the C-terminal domain of an autotransporter capable of translocating, passenger domain to which it is bound;
- wherein the 3′ end of said first nucleic acid sequence is linked to the 5′ end of said second nucleic acid sequence and the 3′ end of said second nucleic acid sequence is linked to the 5′ end of said third nucleic acid sequence.
An expression vector for single-domain recombinant antibodies on the surface of the bacterial outer membrane (OM), characterized in that the expressed antibody is secreted by a transporter domain of an AT.
Dependencies are inferred from the claim text.