Novel glucose dehydrogenase
AssigneeToyo Boseki Kabushiki Kaisha
InventorsHiroshi AIBA, Hiroshi KAWAMINAMI, Takahide KISHIMOTO, Yoshiaki NISHIYA
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Abstract
The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
Claims11
An isolated protein corresponding to any one of the following (a) to (c):
- (a) a protein composed of the amino acid sequence shown in SEQ ID NO:4 and having glucose dehydrogenase activity;
- (b) a protein having 15 or more and 19 or less amino acid deletions in the N terminal side in the amino acid sequence of SEQ ID NO:4 and having glucose dehydrogenase activity;
- and
- (c) a protein having 90% or more homology to the amino acid sequence of SEQ ID NO:4 and having glucose dehydrogenase activity.
An isolated nucleic acid having a base sequence encoding a protein corresponding to any one of the following (a) to (c):
- (a) a protein composed of the amino acid sequence of SEQ ID NO:2 and having glucose dehydrogenase activity;
- (b) a protein having 15 or more and 20 or less amino acid deletions in the N terminal side in the amino acid sequence of SEQ ID NO:2 and having glucose dehydrogenase activity;
- and
- (c) a protein having 90% or more homology to the amino acid sequence of SEQ ID NO:2 and having glucose dehydrogenase activity.
Dependencies are inferred from the claim text.