Method and device for cell counting
OwnerDavid J. Beebe, Nisha A. McConnell +1inventors
InventorsDavid J. Beebe, Nisha A. McConnell, Hongmei Yu
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Abstract
A microfluidic device and method is provided for determining a cell concentration in a sample. The microfluidic device includes a body having a channel therethough that extends along an axis. The channel includes an input and an output, and is at least partially defined by a surface. Indicia overlaps the surface. The channel has a predetermined volume. A portion of the sample is provided in the channel and the cells in the predetermined portions of the channel defined by the indicia are counted.
Claims20
A microfluidic device for determining a cell concentration in a sample, comprising:
- a body having a channel therethough along an axis, the channel including an input and an output and being at least partially defined by a surface;
- and
- indicia overlapping the surface;
- wherein the channel has a predetermined volume.
A method of determining a cell concentration in a sample, comprising the steps of:
- providing a channel in a microfluidic device, the channel having an input, an output and a predetermined volume;
- filling the channel with the sample;
- counting the cells in the channel;
- and
- calculating the cell concentration.
A method of determining a cell concentration in a sample utilizing a microfluidic device having an input and an output;
- comprising the steps of:
- filling the channel with the sample;
- providing indicia for defining predetermined portions of the channel;
- and
- counting the cells in the predetermined portions of the channel.
Dependencies are inferred from the claim text.