Methods of detecting prostate cancer
AssigneeBurnham Institute for Medical Research
InventorsJeffrey W. Smith, Steven J. Kridel, Fumiko T. Axelrod
Life of this patent
Estimating the term…
Abstract
Proteins specific for prostate epithelial cells, normal or neoplastic, are identified and used for diagnosis, development of antibodies, and for evaluating drugs that react with the neoplastic specific proteins. Affinity based probes are used that react specifically with the active site to provide a measure of the enzyme activity of the cells. Prostate epithelial neoplastic cells can be used in screening candidate drugs for their effect in changing the proteome profile as to the serine-threonine hydrolase enzymes, using the affinity based probes for determining the profile.
Claims37
A method for detecting prostate cancer in a patient, comprising:
- providing a cell extract of prostate cells from a patient;
- contacting the cell extract with a probe that measures enzymatic activity of at least one serine hydrolase in the cell extract;
- and
- determining whether the enzymatic activity in the cell extract has changed in comparison to a cell extract from normal prostate cells, wherein a changed enzymatic activity indicates that the prostate cells from the patient are cancerous.
A method for classifying prostate cancer in a patient, comprising:
- providing a cell extract of cancerous prostate cells from a patient;
- contacting the cell extract with a probe that measures enzymatic activity of at least one serine hydrolase in the cell extract;
- and
- determining a pattern of enzymatic activity in the cell extract, wherein said pattern is used to classify the cancer of the prostate cells.
A method for detecting prostate cancer in a patient, comprising:
- providing a cell extract of prostate cells from a patient;
- contacting the cell extract with a probe that measures enzymatic activity of fatty acid synthase in the cell extract;
- and
- determining whether the enzymatic activity is greater in the cell extract in comparison to a cell extract from normal prostate cells, wherein an increased enzymatic activity indicates that the prostate cells from the patient are cancerous.
Dependencies are inferred from the claim text.