Detection of anaplasma platys
AssigneeIDEXX Laboratories, Inc.
InventorsMELISSA JANE BEALL, PHYLLIS IONE TYRRELL, RAMASWAMY CHANDRASHEKAR, JIAYOU LIU
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Abstract
The invention provides compositions and methods for the detection of Anaplasma platys polynucleotides and polypeptides.
Claims20
A method of detecting antibodies that specifically bind an Anaplasma platys polypeptide or an Anaplasma phagocytophilum polypeptide or both, comprising:
- (a) contacting a purified polypeptide comprising at least 15 contiguous amino acids of an amino acid sequence set forth as SEQ ID NO:10, SEQ ID NO:11;
- SEQ ID NO:14, or SEQ ID NO:
- 15, with a test sample, under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies specific for an Anaplasma platys polypeptide or Anaplasma phagocytophilum polypeptide or both are present in the test sample.
A method of detecting an Anaplasma platys infection or Anaplasma phagocytophilum infection or both or exposure to Anaplasma platys or Anaplasma phagocytophilum or both in a subject comprising:
- (a) obtaining a biological sample from the subject;
- (b) contacting a purified polypeptide comprising at least 15 contiguous amino acids of an amino acid sequence set forth as SEQ ID NO:10, SEQ ID NO:11;
- SEQ ID NO:14, or SEQ ID NO:15 with the biological sample under conditions that allow polypeptide/antibody complexes to form;
- and
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that the subject has an Anaplasma platys infection or Anaplasma phagocytophilum infection or both or exposure to Anaplasma platys or Anaplasma phagocytophilum or both.
A method of detecting Anaplasma platys polypeptides or Anaplasma phagocytophilum polypeptides or both in a test sample comprising:
- (a) contacting one or more antibodies that specifically bind to a Anaplasma platys polypeptide with the test sample under conditions that allow polypeptide/antibody complexes to form;
- wherein the Anaplasma platys polypeptide comprises an amino acid sequence set forth SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:14, or SEQ ID NO:15;
- and
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that a Anaplasma platys polypeptide or Anaplasma phagocytophilum polypeptide or both present in the test sample.
Dependencies are inferred from the claim text.