Mammalian T1R3 Sweet Taste Receptors
AssigneesThe Regents of the University of California, Department of Health and Human Services
InventorsIsolde Erlenbach, Nicholas J.P. Ryba, Grace Zhao, Charles S. Zuker
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Abstract
The present invention provides isolated nucleic acid and amino acid sequences of sweet taste receptors, the receptors comprising consisting of a monomer or homodimer of a T1R3 G-protein coupled receptor polypeptide, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sweet and amino acid taste receptors.
Claims20
A method of identifying a compound that modulates taste signal transduction in taste cells, the method comprising the steps of:
- (i) contacting a test compound with an isolated taste receptor comprising at least one T1R3 polypeptide and not comprising T1R1 or T1R2, and wherein the T1R3 polypeptide is at least 90% identical to an amino acid sequence of SEQ ID NO:
- 15, 20, 23, 25, or 31;
- (ii) determining a functional effect of the test compound on the receptor, wherein said functional effect comprises binding to the receptor or having an effect on the activity of the receptor;
- and
- (iii) comparing the functional effect of the test compound to a functional effect of >300 mM natural sugar on said receptor, and identifying the test compound as a compound that modulates taste signal transduction if the effect of the test compound is equal to or greater than the effect of >300 mM natural sugar on said receptor.
A method of identifying a modulator of taste signal transduction in taste cells, the method comprising the steps of:
- (i) contacting a cell or cell membrane with a candidate compound and a natural sugar, wherein the cell or cell membrane comprises a T1R3 polypeptide and does not express T1R1 and T1R2;
- and
- (ii) determining whether the candidate compound modulates an effect of the natural sweetener on the receptor, thereby identifying the candidate compound as the modulator of taste signal transduction.
A method of identifying a compound that modulates taste signal transduction in taste cells, the method comprising the steps of:
- (i) contacting a test compound with an isolated taste receptor comprising at least one T1R3 polypeptide and not comprising T1R1 or T1R2, and wherein the T1R3 polypeptide is at least 90% identical to an amino acid sequence of SEQ ID NO:
- 15, 20, 23, 25, or 31;
- (ii) determining an effect of the test compound on the receptor, wherein said effect comprises binding to the receptor or altering the activity of the receptor;
- and
- (iii) comparing the effect of the test compound to an effect of a natural sweetener on said receptor, and identifying the test compound as a compound that modulates taste signal transduction if the effect of the test compound is equal to or greater than the effect of the natural sweetener on said receptor.
Dependencies are inferred from the claim text.