Lateral Flow Immunoassay With Encapsulated Detection Modality

A lateral flow immunoassay (LFI) device comprising:

• a substrate having a conjugation portion and a test portion;
• encapsulated detection particles conjugated to a detection antibody operatively associated with the conjugation portion;
• and
• capture antibodies operatively associated with the test portion.

A method of performing a lateral flow immunoassay (LFI) comprising:

• providing a substrate;
• providing encapsulated detection particles conjugated to a detection oligonucleotide;
• providing capture oligonucleotides on a test portion of the substrate wherein both the detection oligonucleotides and the capture oligonucleotides are complimentary to a target oligonucleotide of interest;
• binding the encapsulated particles to the target oligonucleotide of interest with the detection oligonucleotide;
• diffusing the fluid sample and encapsulated particles bound to the oligonucleotide of interest through the substrate to the test portion of the substrate;
• capturing the encapsulated particles and oligonucleotide of interest at the test portion of the substrate with the capture oligonucleotides;
• and
• observing the encapsulated particles at the test portion of the substrate.