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US 2009/0155803 A1Published application
Methods for tissue analysis
AssigneeGenentech, Inc.
InventorsRobert L. Cohen, Mary Beth Gardiner, Mark X. Sliwkowski, Gregory T. Stelzer
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Abstract
This invention relates to methods of analyzing a tissue sample from a subject. In particular the invention combines morphological staining and/or immunohistochemistry (IHC) with fluorescence in situ hybridization (FISH) within the same section of a tissue sample. The analysis can be automated or manual. The invention also relates to kits for use in the above methods.
Claims7
A method for detecting the presence of a cellular target protein and a cellular target nucleic acid in a tissue sample, comprising the steps of:
- (a) contacting a section of the tissue sample with an antibody which specifically binds to the target protein;
- (b) determining binding of the antibody to the section of tissue sample;
- (c) hybridizing a labeled nucleic acid probe to the target nucleic acid in the same section of tissue sample;
- (d) detecting the presence of the labeled nucleic acid probe in the section of tissue sample;
- and
- (e) comparing the results of step (b) with the results of step (d).
3 more elements
Dependencies are inferred from the claim text.