Methods for diagnosing pancreatic cancer using reg4 protein
OwnerYusuke Nakamura, Hidewaki Nakagawa +1inventors
InventorsYusuke Nakamura, Hidewaki Nakagawa, Shuichi Nakatsuru
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Abstract
REG4, a new member of REG family was identified as a biomarker of pancreatic adenocarcinoma. The present invention provides sandwich ELISA to detect serum REG4 in patients with resectable pancreatic cancers i.e. PDACs. The present invention also provides a method for diagnosing a pancreatic cancer using REG4 as a serological marker.
Claims20
A method for diagnosing pancreatic cancer in a subject, comprising the steps of:
- (a) providing a blood sample from a subject to be diagnosed;
- (b) determining a level of REG4 in the blood sample;
- (c) comparing the REG4 level determined in step (b) with that of a normal control, wherein a high REG4 level in the blood sample, compared to the normal control, indicates that the subject suffers from pancreatic cancer.
The method of claim. 3, wherein the REG4 protein is detected by immunoassay.
An immunoassay reagent for detecting REG4 in a blood sample, wherein the reagent comprises an anti-REG4 antibody.
A kit for detecting a pancreatic cancer, wherein the kit comprises:
- (i) an immunoassay reagent for determining a level of REG4 in a blood sample;
- and
- (ii) a positive control sample for REG4.
A positive control blood sample for detecting a pancreatic cancer, wherein the blood sample comprises more than normal level of both of REG4 and CA19-9.
An anti-REG4 monoclonal antibody comprises a VH and VL chain, each VH and VL chain comprising CDR amino acid sequences designated CDR1, CDR2 and CDR3 separated by framework amino acid sequences, the amino acid sequence of each CDR in each VH and VL chain is selected from the group consisting of:
- VH CDR1:
- SEQ ID NO:
- 18
- VH CDR2:
- SEQ ID NO:
- 20
- VH CDR3:
- SEQ ID NO:
- 22
- VL CDR1:
- SEQ ID NO:
- 26
- VL CDR2:
- SEQ ID NO:
- 28 and
- VL CDR3:
- SEQ ID NO:
- 30,
- or a fragment comprising antigen binding region thereof.
Dependencies are inferred from the claim text.