Back to results
US 2009/0155786 A1Published application
Compositions and methods for detecting markers of cancer
AssigneePresident and Fellows of Harvard College
InventorsCarmen J. Marsit, Margaret R. Karagas, Angeline S. Andrew, Mei Liu, Karl T. Kelsey
Life of this patent
Estimating the term…
Abstract
Disclosed herein are compositions and methods for detecting one or more markers indicative of cancer. In one instance the marker is one or more methylated genes, such as SFRPs. In another instance the marker is an altered protein, such as p53.
Claims20
A method of detecting one or more markers indicative of cancer in an individual, comprising:
- (a) obtaining a suitable biological sample;
- (b) isolating nucleic acid from said biological sample of (a);
- (c) subjecting said isolated nucleic acid of (b) to methylation-specific PCR;
- and
- (d) detecting said one or more markers in said individual.
2 more elements
A method of detecting one or more markers indicative of cancer in an individual, comprising:
- (a) obtaining a suitable biological sample;
- (b) isolating (i) nucleic acid and (ii) tissue sample from said biological sample of (a);
- (c) subjecting said isolated nucleic acid of (b) to methylation-specific PCR;
- (d) subjecting said tissue sample of (b) to immunohistochemistry analysis;
- and
- (d) detecting said one or more markers in said individual.
3 more elements
Dependencies are inferred from the claim text.