High-Risk Human Papillomavirus Detection

A composition comprising a sample and at least one oligonucleotide that comprises a nucleic acid with a sequence selected from the group consisting of:

• SEQ ID NOS:
• 1-31, a substantially identical variant thereof wherein the variant has at least 90% sequence identity to one of SEQ ID NOS:
• 1-31, and complements of SEQ ID NOS:
• 1-31 and the variant, which oligonucleotide consists of 100 or fewer nucleotides.

A method of determining a presence of at least one high-risk human papillomavirus (HPV) type in a sample, the method comprising:

• (a) contacting nucleic acids and/or amplicons thereof from the sample with at least one oligonucleotide that comprises a nucleic acid with a sequence selected from the group consisting of:
• SEQ ID NOS:
• 1-31 and complements of SEQ ID NOS:
• 1-31, which oligonucleotide consists of 100 or fewer nucleotides;
• and,
• (b) monitoring binding between the nucleic acids and/or amplicons thereof, and the oligonucleotide, wherein detectable binding between the nucleic acids and/or amplicons thereof, and the oligonucleotide, determines the presence of the high-risk HPV type in the sample.

A method of determining a presence of at least one high-risk human papillomavirus (HPV) type in a sample, the method comprising:

• (a) contacting nucleic acids and/or amplicons thereof from the sample with at least one oligonucleotide that comprises a nucleic acid having at least 90% sequence identity to one of SEQ ID NOS:
• 1-31 and complements of SEQ ID NOS:
• 1-31, which oligonucleotide consists of 100 or fewer nucleotides;
• and,
• (b) monitoring binding between the nucleic acids and/or amplicons thereof, and the oligonucleotide, wherein detectable binding between the nucleic acids and/or amplicons thereof, and the oligonucleotide, determines the presence of the high-risk HPV type in the sample.

A kit, comprising:

• (a) at least one oligonucleotide that comprises a nucleic acid with a sequence selected from the group consisting of:
• SEQ ID NOS:
• 1-31, a substantially identical variant thereof wherein the variant has at least 90% sequence identity to one of SEQ ID NOS:
• 1-31, and complements of SEQ ID NOS:
• 1-31 and the variant, which oligonucleotide consists of 100 or fewer nucleotides;
• and,
• (b) instructions for determining a presence of at least one high-risk human papillomavirus (HPV) type in a sample by monitoring binding between nucleic acids and/or amplicons thereof from the sample and the oligonucleotide.

A system for detecting at least one high-risk human papillomavirus (HPV) type in a sample, comprising:

• (a) at least one oligonucleotide that comprises a nucleic acid with a sequence selected from the group consisting of:
• SEQ ID NOS:
• 1-31, a substantially identical variant thereof wherein the variant has at least 90% sequence identity to one of SEQ ID NOS:
• 1-31, and complements of SEQ ID NOS:
• 1-31 and the variant, which oligonucleotide consists of 100 or fewer nucleotides;
• (b) at least one detector that detects binding between nucleic acids and/or amplicons thereof from the sample and the oligonucleotide;
• and,
• (c) at least one controller operably connected to the detector, which controller comprises one or more instructions sets that correlate the binding detected by the detector with a presence of the high-risk HPV type in the sample.

A system, comprising:

• (a) computer or computer readable medium comprising a data set that comprises at least one character string that corresponds to at least one sequence selected from the group consisting of:
• SEQ. ID NOS. 1-31 and complements thereof;
• and,
• (b) an automatic synthesizer coupled to an output of the computer or computer readable medium, which automatic synthesizer accepts instructions from the computer or computer readable medium, which instructions direct synthesis of one or more nucleic acids that correspond to one or more character strings in the data set.