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AssigneesMelbourne Health, Austin and Repatriation Medical Centre, Central Sydney Area Health Service
InventorsAngeline Ingrid BARTHOLOMEUSZ, Stephen Alister Locarnini, Anna Ayres, Margaret Rose Littlejohn, Geoffrey William McCaughan, Peter William Angus
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The present invention relates generally to viral variants exhibiting reduced sensitivity to agents and in particular nucleoside analogues. More particularly, the present invention is directed to hepatitis B virus variants exhibiting complete or partial resistance to nucleoside analogues. The variants may also comprise corresponding mutations affecting immunological interactivity to viral surface components. The present invention further contemplates assays for detecting such viral variants which assays are useful in monitoring anti-viral therapeutic regimes and in developing new or modified vaccines directed against viral agents and in particular hepatitis B virus variants. The present invention also contemplates the use of the viral variants to screen for agents capable of inhibiting infection, replication and/or release of the virus.
An isolated HBV variant comprising a mutation in its DNA polymerase gene that results in a S548C substitution in its DNA polymerase, wherein the site of said substitution corresponds to position 548 of the sequence set forth in FIG. 2 (SEQ ID NO:7).
A method for determining the potential for an HBV to exhibit reduced sensitivity to a nucleoside analogue, said method comprising isolating DNA or corresponding mRNA from said HBV and screening for a mutation in the nucleotide sequence encoding HBV DNA polymerase resulting in a S548C substitution in its DNA polymerase, wherein the site of said substitution corresponds to position 548 of the sequence set forth in FIG. 2 (SEQ ID NO:7), and wherein the presence of such a mutation is an indication of the likelihood of resistance to said nucleoside analogue.
Dependencies are inferred from the claim text.