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US 2009/0155766 A1Published application
Methods for detecting estrone by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsMildred M. Goldman, Nigel J. Clarke, Richard E. Reitz
Life of this patent
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Abstract
Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.
Claims32
A method for determining the amount of estrone in a body fluid sample said method comprising:
- (a) purifying estrone from said body fluid sample by liquid chromatography;
- (b) ionizing the purified estrone from said body fluid sample to produce one or more ions detectable by tandem mass spectrometry;
- and
- (c) detecting the amount of the estrone ion(s) by tandem mass spectrometry, wherein the amount of the estrone ion(s) is related to the amount of estrone in said body fluid sample;
- wherein the estrone is not derivatized prior to mass spectrometry.
2 more elements
A method for determining the amount of estrone in a body fluid sample by tandem mass spectrometry said method comprising:
- (a) purifying estrone from said body fluid sample by liquid chromatography;
- (b) generating a precursor ion of said estrone having a mass/charge ratio of 269.07±0.5;
- (c) generating one or more fragment ions of said precursor ion, wherein at least one of said one or more fragment ions comprise an ion fragment having a mass/charge ratio of 143.02±0.5;
- and
- (d) detecting the amount of one or more of said ions generated in step (b) or (c) or both and relating the detected ions to the amount of said estrone in said body fluid sample.
2 more elements
A method for determining the amount of estrone in a body fluid sample said method comprising:
- (a) acidifying said body fluid sample with an agent in an amount sufficient to free estrone from a protein that may be present in said body fluid sample;
- (b) purifying estrone from said body fluid sample by liquid chromatography;
- (c) ionizing the purified estrone from said body fluid sample to produce one or more ions detectable by tandem mass spectrometry;
- and
- (d) detecting the amount of the estrone ion(s) by tandem mass spectrometry in negative ion mode, wherein the amount of the estrone ion(s) is related to the amount of estrone in said body fluid sample.
2 more elements
Dependencies are inferred from the claim text.