Method of Cell Injection into Biotissue and Apparatus Therefor
AssigneesJapan AS Represented By President of National Cardiovascular Center, Nipro Corporation
InventorsToshiya Fujisato, Akio Kishida, Soichiro Kitamura
Life of this patent
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Abstract
A method of injecting cells into a biological tissue comprising thrusting injection needle (200) under microvibration into tissue (5) secured by suction to tissue suction means (210) and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.
Claims25
A method for injecting cells into elastically deformable biological tissues comprising:
- adhering the tissue at least in part on a support member;
- positioning an injection needle at a desired site of the tissue;
- generating microvibration in said needle;
- piercing the tissue with said needle at said desired site;
- and
- injecting a cell suspension through said needle.
A system for injecting cells into elastically deformable biological tissue comprising:
- means for adhering the tissue at least in part on a supporting member;
- means for positioning an injection needle at a desired site of the tissue;
- means for generating microvibration in said needle;
- means for piercing tissue with said needle at said desired site;
- and
- means for injection a cell suspension through said needle.
A method for preparing a transplantable biological tissue segment comprising the steps of:
- (a) providing a biological tissue segment suitable for transplantation;
- (b) applying said tissue on a first face of a support member defining a plurality of throughholes;
- (c) holding said tissue by applying a negative pressure on a second face of said support member opposite to the first face;
- (d) generating microvibration in an injection needle;
- (e) piercing said tissue with said injection needle at a desired point to a predetermined depth by advancing the needle through the interface between the tissue and the first face of said support member;
- (f) infusing a predetermined volume of a cell suspension;
- and
- (g) repeating the steps (a) to (f) at different points of the tissue other than the first point sequentially.
A system for injecting cells into a biological tissue segment comprising;
- an injection needle;
- means for generating microvibration in said needle;
- means for holding said tissue on a first face of a support member, said support member defining a plurality of throughholes and slidably supporting said needle therethrough;
- means for alternately applying a negative pressure and a positive pressure in said throughholes;
- means for advancing and retracting the distal end of said needle a predetermined distance across the first face of said support member;
- means for infusing a predetermined volume of a cell suspension into the tissue through said needle when the needle is extended beyond said first face of said support member;
- and
- means for positioning and repositioning said needle, when the needle is retracted from the extended position, to a series of point on the tissue according to their X and Y coordinates.
Dependencies are inferred from the claim text.