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US 2009/0155413 A1Published application
Enzyme
OwnerOle Sibbesen, Jens Frisbaek Sorenseninventors
Life of this patent
Estimating the term…
Abstract
The present invention relates to a variant xylanase polypeptide, or fragment thereof having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with parent enzyme.
Claims86
1-22. (canceled)
A method of degrading or modifying a plant cell wall which method comprises contacting said plant cell wall with a polypeptide, wherein said polypeptide is a variant xylanase polypeptide, or fragment thereof having xylanase activity;
- wherein said variant xylanase polypeptide, or fragment thereof having xylanase activity, comprises one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme.
A method of processing a plant material which method comprises contacting said plant material with a polypeptide, wherein said polypeptide is a variant xylanase polypeptide, or fragment thereof having xylanase activity;
- wherein said variant xylanase polypeptide, or fragment thereof having xylanase activity, comprises one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme.
A method of baking or processing cereals or starch production or processing wood or enhancing the bleaching of wood pulp which method comprises the use of a polypeptide, wherein said polypeptide is a variant xylanase polypeptide, or fragment thereof having xylanase activity;
- wherein said variant xylanase polypeptide, or fragment thereof having xylanase activity, comprises one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme.
A nucleotide sequence encoding a variant polypeptide, wherein said polypeptide is a variant xylanase polypeptide, or fragment thereof having xylanase activity;
- wherein said variant xylanase polypeptide, or fragment thereof having xylanase activity, comprises one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme.
An isolated variant polypeptide or fragment thereof having at least 40% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package, and having xylanase activity, comprising two or more amino acid modifications, wherein said amino acid modifications are at two or more of positions 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ ID NO:
- 1, or their equivalent positions in other family 11 xylanases, such that the polypeptide or fragment thereof has altered sensitivity to a xylanase inhibitor as compared with the parent xylanase, wherein one of said modifications is at position 11, wherein the variant polypeptide is a family 11 xylanase.
An isolated variant polypeptide or fragment thereof having at least 50% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package, and having xylanase activity, comprising two or more amino acid modifications, wherein said amino acid modifications are at two or more of positions 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ ID NO:
- 1, or their equivalent positions in other family 11 xylanases, such that the polypeptide or fragment thereof has altered sensitivity to a xylanase inhibitor as compared with the parent xylanase, wherein one of said modifications is at position 11, wherein the variant polypeptide is a family 11 xylanase.
An isolated variant polypeptide or fragment thereof having at least 60% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package, and having xylanase activity, comprising two or more amino acid modifications, wherein said amino acid modifications are at two or more of positions 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ ID NO:
- 1, or their equivalent positions in other family 11 xylanases, such that the polypeptide or fragment thereof has altered sensitivity to a xylanase inhibitor as compared with the parent xylanase, wherein one of said modifications is at position 11, wherein the variant polypeptide is a family 11 xylanase.
A variant xylanase polypeptide, or fragment thereof having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from SEQ ID No. 9.
3 more elements
A variant xylanase polypeptide, or fragment thereof having at least 40% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from SEQ ID No. 9.
4 more elements
A variant xylanase polypeptide, or fragment thereof having at least 50% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from SEQ ID No. 9.
4 more elements
A variant xylanase polypeptide, or fragment thereof having at least 60% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from SEQ ID No. 9.
4 more elements
A variant xylanase polypeptide, or fragment thereof having at least 80% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from SEQ ID No. 9.
4 more elements
A variant xylanase polypeptide, or fragment thereof having at least 40% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from any of the xylanase sequences presented in FIG. 2 or referred to in the following Table:
- Aspergillus niger Xyn A
- Aspergillus kawachii Xyn C
- Aspergillus tubigensis Xyn A
- Bacillus circulans Xyn A
- Bacillus pumilus Xyn A
- Bacillus subtilis Xyn A
- Cellulomonas fimi Xyn D
- Chainia spp. Xyn
- Clostridium acetobutylicum Xyn B
- Clostridium stercorarium Xyn A
- Fibrobacter succinogenes Xyn C
- Neocallimastix patriciarum Xyn A
- Nocardiopsis dassonvillei Xyn II
- Ruminococcus flavefaciens Xyn A
- Schizophyllum commune Xyn
- Streptomyces lividans Xyn B
- Streptomyces lividans Xyn C
- Streptomyces sp. No. 36a Xyn
- Streptomyces thermoviolaceus Xyn II
- Thermomonospora fusca Xyn A
- Trichoderma harzianum Xyn
- Trichoderma reesei Xyn I
- Trichoderma reesei Xyn II
- Trichoderma viride Xyn
28 more elements
A variant xylanase polypeptide, or fragment thereof having at least 50% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from any of the xylanase sequences presented in FIG. 2 or referred to in the following Table:
- Aspergillus niger Xyn A
- Aspergillus kawachii Xyn C
- Aspergillus tubigensis Xyn A
- Bacillus circulans Xyn A
- Bacillus pumilus Xyn A
- Bacillus subtilis Xyn A
- Cellulomonas fimi Xyn D
- Chainia spp. Xyn
- Clostridium acetobutylicum Xyn B
- Clostridium stercorarium Xyn A
- Fibrobacter succinogenes Xyn C
- Neocallimastix patriciarum Xyn A
- Nocardiopsis dassonvillei Xyn II
- Ruminococcus flavefaciens Xyn A
- Schizophyllum commune Xyn
- Streptomyces lividans Xyn B
- Streptomyces lividans Xyn C
- Streptomyces sp. No. 36a Xyn
- Streptomyces thermoviolaceus Xyn II
- Thermomonospora fusca Xyn A
- Trichoderma harzianum Xyn
- Trichoderma reesei Xyn I
- Trichoderma reesei Xyn II
- Trichoderma viride Xyn
28 more elements
A variant xylanase polypeptide, or fragment thereof having at least 60% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from any of the xylanase sequences presented in FIG. 2 or referred to in the following Table:
- Aspergillus niger Xyn A
- Aspergillus kawachii Xyn C
- Aspergillus tubigensis Xyn A
- Bacillus circulans Xyn A
- Bacillus pumilus Xyn A
- Bacillus subtilis Xyn A
- Cellulomonas fimi Xyn D
- Chainia spp. Xyn
- Clostridium acetobutylicum Xyn B
- Clostridium stercorarium Xyn A
- Fibrobacter succinogenes Xyn C
- Neocallimastix patriciarum Xyn A
- Nocardiopsis dassonvillei Xyn II
- Ruminococcus flavefaciens Xyn A
- Schizophyllum commune Xyn
- Streptomyces lividans Xyn B
- Streptomyces lividans Xyn C
- Streptomyces sp. No. 36a Xyn
- Streptomyces thermoviolaceus Xyn II
- Thermomonospora fusca Xyn A
- Trichoderma harzianum Xyn
- Trichoderma reesei Xyn I
- Trichoderma reesei Xyn II
- Trichoderma viride Xyn
28 more elements
A variant xylanase polypeptide, or fragment thereof having at least 80% homology to SEQ ID NO:
- 1 as determined by using the GCG Wisconsin Bestfit package and having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from any of the xylanase sequences presented in FIG. 2 or referred to in the following Table:
- Aspergillus niger Xyn A
- Aspergillus kawachii Xyn C
- Aspergillus tubigensis Xyn A
- Bacillus circulans Xyn A
- Bacillus pumilus Xyn A
- Bacillus subtilis Xyn A
- Cellulomonas fimi Xyn D
- Chainia spp. Xyn
- Clostridium acetobutylicum Xyn B
- Clostridium stercorarium Xyn A
- Fibrobacter succinogenes Xyn C
- Neocallimastix patriciarum Xyn A
- Nocardiopsis dassonvillei Xyn II
- Ruminococcus flavefaciens Xyn A
- Schizophyllum commune Xyn
- Streptomyces lividans Xyn B
- Streptomyces lividans Xyn C
- Streptomyces sp. No. 36a Xyn
- Streptomyces thermoviolaceus Xyn II
- Thermomonospora fusca Xyn A
- Trichoderma harzianum Xyn
- Trichoderma reesei Xyn I
- Trichoderma reesei Xyn II
- Trichoderma viride Xyn
28 more elements
A variant xylanase polypeptide, or fragment thereof having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor as compared with the parent xylanase enzyme;
- wherein the variant polypeptide is derived from a family 11 xylanase;
- wherein said amino acid modification is of two or more surface amino acid residues;
- wherein said amino acid modification is at an equivalent position to one or more of amino acid residues numbers:
- 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- wherein one of said modifications is at an equivalent position to amino acid residue number 11 of the B. subtilis amino acid sequence shown as SEQ I.D. No. 1;
- and wherein said polypeptide is derivable from any of the xylanase sequences presented in FIG. 2 or referred to in the following Table:
- Aspergillus niger Xyn A
- Aspergillus kawachii Xyn C
- Aspergillus tubigensis Xyn A
- Bacillus circulans Xyn A
- Bacillus pumilus Xyn A
- Bacillus subtilis Xyn A
- Cellulomonas fimi Xyn D
- Chainia spp. Xyn
- Clostridium acetobutylicum Xyn B
- Clostridium stercorarium Xyn A
- Fibrobacter succinogenes Xyn C
- Neocallimastix patriciarum Xyn A
- Nocardiopsis dassonvillei Xyn II
- Ruminococcus flavefaciens Xyn A
- Schizophyllum commune Xyn
- Streptomyces lividans Xyn B
- Streptomyces lividans Xyn C
- Streptomyces sp. No. 36a Xyn
- Streptomyces thermoviolaceus Xyn II
- Thermomonospora fusca Xyn A
- Trichoderma harzianum Xyn
- Trichoderma reesei Xyn I
- Trichoderma reesei Xyn II
- Trichoderma viride Xyn
27 more elements
Dependencies are inferred from the claim text.