Method of cross-linking hyaluronic acid with divinulsulfone
AssigneeNovozymes Biopolymer
InventorsFanny Longin, Khadija Schwach-Abdellaoui
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Abstract
The present invention relates to methods of producing a homogenous hydrogel comprising hyaluronic acid, or salt thereof, crosslinked with divinylsulfone (DVS), said method comprising the steps of (a) providing an alkaline solution of hyaluronic acid, or salt thereof; (b) adding DVS to the solution of step (a), whereby the hyaluronic acid, or salt thereof, is crosslinked with the DVS to form a gel; (c) treating the gel of step (b) with a buffer, wherein the gel swells and forms a hydrogel comprising hyaluronic acid, or salt thereof, crosslinked with DVS.
Claims21
1-41. (canceled)
A method of producing a hydrogel comprising hyaluronic acid or a salt thereof, crosslinked with divinylsulfone (DVS) said method comprising the steps of:
- (a) adding DVS to an alkaline solution of the hyaluronic acid or salt thereof, whereby the hyaluronic acid or salt thereof, is crosslinked with the DVS to form a gel;
- and
- (b) treating the gel of step (a) with a buffer, wherein the gel swells and forms a hydrogel comprising the hyaluronic acid or salt thereof, crosslinked with DVS.
A hydrogel comprising hyaluronic acid or a salt thereof, crosslinked with divinylsulfone (DVS), which is sufficiently homogeneous to be injected from a 1 ml syringe through a 27G½ needle over a distance of 55 mm at a speed of 12.5 mm/min with a stable injection force, which after the initial 40 seconds of the injection, and until the syringe is empty, varies no more than about 5 Newton (N).
Dependencies are inferred from the claim text.