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US 2009/0155306 A1Published application
Pamps, pathogen associated molecular patterns
AssigneeNovartis AG
InventorsJeffrey Ulmer, Nicholas Valiante, Feng Xu, Claudio Donati, Antonello Covacci
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Abstract
A method for identifying a polypeptide which acts as an adjuvant in a host organism. The invention further provides adjuvant compositions comprising said polypeptides and optionally further comprising an antigen.
Claims16
A method for identifying a polypeptide which acts as an adjuvant in a host organism comprising the steps of:
- a) generating protein families by grouping together amino acid sequences from at least a different pathogenic organisms, which sequences share a BLAST alignment with an E-score less than 1E-05;
- b) selecting a protein family of step a) wherein:
- (i) the family includes sequences from at least b different pathogenic organisms, and
- (ii) at least one of the proteins in the family does not share a BLAST alignment with an E-score smaller than 1E-05 with amino acid sequences from a chosen non-pathogenic organism;
- c) determining the sequence motifs from the resulting families of step b) that are conserved within the family;
- and
- d) selecting polypeptide sequences that comprise the motifs of step c),
- wherein:
- a is at least 60 and b is at least 30, where b≦a.
6 more elements
A polypeptide comprising:
- (i) any one of the amino acid sequences listed in Table 3;
- (ii) an amino acid sequence having at least 85% sequence identity to an amino acid sequence from Table 3 and/or comprising an amino acid sequence consisting of a fragment of at least 5 contiguous amino acids from an amino acid sequence from Table 3;
- or (iii) an amino acid sequence listed in Table 3, except that the amino acid sequence contains one or more variations.
A polymer comprising any one of the sequences listed in Table 3, wherein (a) the polymer comprises monomers selected from the group consisting of:
- L-amino acids;
- D-amino acids;
- and amino acid mimetics, and/or (b) the bonds between monomers are not peptide bonds, wherein said polymer does not consist of:
- a chain of L-amino acids joined by peptide bonds to form a linear unbranched polypeptide chain.
1 more element
Dependencies are inferred from the claim text.