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US 2009/0155304 A1Published application
Modified Cyanovirin-N Polypeptide
AssigneeOsel, Inc.
InventorsXiaowen Liu, Kirsten Essenmacher, David A. Simpson, Qiang Xu
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AssigneeOsel, Inc.
InventorsXiaowen Liu, Kirsten Essenmacher, David A. Simpson, Qiang Xu
Estimating the term…
This disclosure relates to a modified version of cyanovirin-N protein and a transformed Lactobacillus bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformed Lactobacillus bacterium. In addition, this disclosure describes two novel promoters originated from L. jensenii, which are capable of directing a high level of gene expression in Lactobacillus bacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modified Lactobacillus bacterium containing the expression cassette, a method for recombinant gene expression in Lactobacillus bacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.
A Lactobacillus bacterium expressing a modified polypeptide comprising a core amino acid sequence identical to the amino acid sequence of the unmodified polypeptide and an additional amino acid sequence immediately to the N-terminus of the core amino acid sequence, wherein the additional amino acid sequence consists of two to twenty amino acids, among which at least one of the first two amino acids is identical to its corresponding amino acid in the first two amino acids of a mature secreted Lactobacillus protein following the cleavage of a signal sequence, wherein the modified polypeptide is derived from a precursor polypeptide following the cleavage of the signal sequence of the secreted Lactobacillus protein, and wherein the modified polypeptide retains the same biological activity as the unmodified polypeptide.
A modified polypeptide comprising a core amino acid sequence identical to the amino acid sequence of the unmodified polypeptide and an additional amino acid sequence immediately to the N-terminus of the core amino acid sequence, wherein the additional amino acid sequence consists of two to twenty amino acids, among which at least one of the first two amino acids is identical to its corresponding amino acid in the first two amino acids of a mature secreted Lactobacillus protein following the cleavage of a signal sequence, wherein the modified polypeptide is derived from a precursor polypeptide following the cleavage of the signal sequence of the secreted Lactobacillus protein, and wherein the modified polypeptide retains the same biological activity as the unmodified polypeptide.
11-12. (canceled)
14-19. (canceled)
Dependencies are inferred from the claim text.