Pseudoinfectious flavivirus and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsPeter W. Mason, Elena Frolova, Ilya Frolov
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Abstract
The present invention discloses a replication-deficient pseudoinfective virus belonging to the Flaviviridae family that lack the capsid gene, where the replication-deficient pseudoinfective virus propagates only in cells expressing the capsid or capsid, prM and envelope protein of the flavivirus. The present also discloses the method of producing such viruses on a large scale and the use of these pseudoinfective viruses as vaccines for preventing diseases caused by infections of humans or animals by the viruses belonging to this family.
Claims35
A replication-deficient pseudoinfectious virus of Flaviviridae family, comprising:
- a deletion in the nucleotide sequence encoding capsid (C) protein such that the deletion does not disrupt the RNA sequence required for genome cyclization, the signal sequence for prM protein that is required for the proper maturation of prM/M or a combination thereof, wherein said replication-deficient pseudoinfectious virus replicate only in cells expressing C protein or C, prM, envelope protein, mutated C protein, mutated prM, mutated envelope protein or combinations thereof of a virus of the Flaviviridae family.
A method of producing a replication-deficient pseudoinfectious virus of Flaviviridae family, comprising;
- generating a replication-deficient pseudoinfectious virus of Flaviviridae family that comprises a deletion in the capsid gene such that the deletion does not disrupt the RNA sequence required for genome cyclization, the signal sequence for prM protein that is required for the proper maturation of prM/M or a combination thereof;
- generating a cell line that expresses capsid protein or capsid, prM, E protein, mutated capsid protein, mutated prM, mutated E protein or combinations thereof of the virus of the Flaviviridae family, wherein the cell line provides high levels of the proteins of needed for propagation of the replication-deficient pseudoinfectious virus of Flaviviridae family;
- and
- infecting the cell line with the pseudoinfectious virus of the Flaviviridae family, thereby producing the replication-deficient pseudoinfectious virus of the Flaviviridae family.
Dependencies are inferred from the claim text.