Chimeric anti-vegf-d antibodies and humanized anti-vegf-d antibodies and methods of using same
AssigneeVegenics Limited
InventorsMarc Gregory Achen, Steven Alan Stacker, Christoph Renner
Life of this patent
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Abstract
The present invention relates to materials and methods for modulating angiogenesis and lymphangiogenesis. The compositions of the invention provide chimeric and/or humanized VEGF-D antibody substances, antibodies, polypeptides and fragments thereof useful for modulating angiogenesis and lymphangiogenesis in a subject.
Claims32
A chimeric or humanized antibody substance according to claim 2 comprising:
- (a) an antibody that specifically binds Vascular Endothelial Growth Factor-D (VEGF-D), comprising
- i) a light chain variable region comprising complementarity determining regions (L-CDR), wherein at least one of said L-CDR comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 47-49;
- ii) a heavy chain variable region comprising complementarity determining regions (H-CDR), wherein at least one of said H-CDR comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 50-52;
- iii) a human antibody light chain constant region;
- and
- iv) a human antibody heavy chain constant region;
- or
- (b) a fragment of (a) that binds VEGF-D.
A chimeric or humanized antibody substance, antibody, or antibody fragment that binds VEGF-D, or a polypeptide comprising an antigen binding region of a VEGF-D antibody, that comprises an amino acid sequence at least 90% identical to at least one polypeptide selected from the group consisting of SEQ ID NO:
- 37, SEQ ID NO:
- 39, SEQ ID NOs:
- 47-49 and SEQ ID NOs:
- 50-52.
(canceled)
-13. (canceled)
(canceled)
-19. (canceled)
-22. (canceled)
A purified polypeptide according to claim 24 that binds VEGF-D, comprising an antigen binding region of a VEGF-D antibody, wherein the antigen binding region comprises an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:
- 37 or SEQ ID NO:
- 39, and wherein the antibody is humanized.
(canceled)
28-31. (canceled)
34-44. (canceled)
An isolated polynucleotide according to claim 51 comprising a nucleotide sequence that encodes a light chain polypeptide of a chimeric or humanized VEGF-D antibody or antigen-binding fragment thereof, wherein the light chain polypeptide comprises L-CDR sequences set forth in SEQ ID NOs:
- 47-49.
(canceled)
An isolated polynucleotide according to claim 51 comprising a nucleotide sequence that encodes a heavy chain polypeptide of a chimeric or humanized VEGF-D antibody or antigen-binding fragment thereof, wherein the heavy chain polypeptide comprises H-CDR sequences set forth in SEQ ID NOs:
- 50-52.
48-50. (canceled)
52-59. (canceled)
61-65. (canceled)
Dependencies are inferred from the claim text.