Antibodies Specific For Soluble Amyloid Beta Peptide Protofibrils and Uses Thereof

A method of synthesizing pure Aβ42arc protofibril antigen, comprising the steps of:

• dissolving an Aβ42arc peptide by using a dissociating agent, to achieve a monodisperse solution,
• neutralizing the solution by PBS (pH 7-8) or similar biocompatible buffers, to achieve a physiological solution,
• stabilizing the spontaneously formed protofibrils by keeping them at below 20° C.,
• centrifuging the protofibrils, at the same temperature as in the stabilising step, at a sufficient speed and time to sediment Aβ42 arc fibrils,
• assessing the purity of the protofibril preparation by HPLC to control that the purity is >95%.

A method to synthesize pure wild type Aβ42 protofibril antigen, comprising the steps of:

• dissolving an Aβ42arc peptide by using a dissociating agent, to achieve a monodisperse solution,
• neutralizing the solution by PBS (pH 7-8) or similar biocompatible buffers, to achieve a physiological solution,
• incubating the neutralized Aβ42wt peptide solution at an elevated protein concentration between 1-1000 uM, for 6 hours or longer at 20-40° C., to form Aβ42wt protofibrils,
• diluting the protofibrils to 1-500 uM,
• centrifuging at sufficient speed and time to sediment Aβ42 wt fibrils,
• assessing the purity of the protofibril preparation by HPLC to control that the purity is >95%.