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US 2009/0155239 A1Published application
Novel Protease, Microorganism Producing the Same, and Application Thereof
AssigneeSodx Co., Ltd.
InventorTakumi Nakamura
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Abstract
An object of the present invention are to provide a protease that is stable in a wide pH range from acidic to alkaline, and that has excellent thrombolytic activity; a protease-producing microorganism that produces the above protease; and a process for producing the protease. By culturing a novel filamentous fungus belonging to the genus Fusarium (Fusarium sp. strain BLB), a protease that is stable in a wide pH range from acidic to alkaline and that has excellent thrombolytic activity, is formed and accumulated in the culture medium, and recovered.
Claims18
A protease having the following properties:
- (1) activity/substrate specificity:
- having fibrinolytic activity, and degrading activity on synthetic substrates H-D-Ile-Pro-Arg-pNA, H-D-Val-Leu-Lys-pNA, and Bz-L-Arg-pNA;
- (2) active pH and optimum pH:
- being active at least within a pH range of 6.5 to 11.5, and being optimally active at about pH 8.5 to about 9.5;
- (3) pH stability:
- being stable at least within a pH range of 2.5 to 11.5 under treatment conditions of 4° C. and 20 hours;
- (4) active temperature and optimum temperature:
- being active at least within a temperature range of 30 to 50° C., and being optimally active at about 45 to about 50° C.;
- (5) temperature stability:
- being stable at least about 55° C. under treatment conditions of pH 5 and 10 minutes;
- (6) molecular weight:
- having an estimated molecular weight of about 27000 on SDS-PAGE;
- (7) inhibitory properties:
- not being inhibited by 0.01 mg/ml SBTI but being inhibited by 1 mM PMSF and 0.1 mM DFP.
11 more elements
Dependencies are inferred from the claim text.