Compositions and methods for growth of embryonic stem cells
OwnerAlberto Hayek, Gillian Beattie +1inventors
InventorsAlberto Hayek, Gillian Beattie, Ana Lopez
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Abstract
The present invention relates to methods, compositions, and kits for maintenance of the undifferentiated state and/or pluripotency in stem cells, including embryonic stem (ES) cells. It also relates to stem cells that have been maintained in an undifferentiated state. The present invention provides these aspects using culture medium enriched with a TGFβ family member protein, an FGF family member protein, and/or nicotinamide, without using fibroblast feeder layers, conditioned media, or leukemia inhibitory factor.
Claims66
A method for maintenance of an undifferentiated stem cell, said method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state for a sufficient amount of time to achieve a desired result.
A composition comprising a) a culture medium and b) a TGFβ family member, an FGF family member, NIC, or a combination of two or more of these.
A composition comprising a combination of two or more of a) at least one purified TGFβ family member protein, b) at least one purified FGF family member protein, and 3) purified NIC.
An undifferentiated, pluripotent stem cell derived from a culture that has been passaged at least 10 times.
A stem cell produced by a method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state.
A kit comprising two or more of the following:
- a) a TGFβ family member,
- b) an FGF family member,
- c) NIC,
- d) a stem cell, and
- e) stem cell culture media.
A method for maintenance of an undifferentiated stem cell, said method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state for a sufficient amount of time to achieve a desired result, wherein the stem cell is not also exposed to a feeder cell, conditioned media, or leukemia inhibitory factor.
A composition comprising a) a culture medium and b) a TGFβ family member, an FGF family member, NIC, or a combination of two or more of these, wherein said composition does not comprise feeder cells, conditioned media, or LIF.
A differentiated cell derived from a stem cell grown or maintained by a method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state.
An embryonic stem cell grown or maintained by a method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state.
A pharmaceutical composition comprising a stem cell grown or maintained by a method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state.
A cell derived from a stem cell grown or maintained by a method comprising exposing a stem cell to a member of the transforming growth factor-beta (TGFβ) family of proteins, a member of the fibroblast growth factor (FGF) family of proteins, or nicotinamide (NIC) in an amount sufficient to maintain the cell in an undifferentiated state
Dependencies are inferred from the claim text.