Lactobacillus acidophilus nucleic acids and uses thereof
AssigneeNorth Carolina State University
InventorsAndrea Azcarate-Peril, Eric Altermann, Todd Klaenhammer
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Abstract
Lactobacillus acidophilus NCFM nucleic acid molecules, polypeptides, fragments and variants thereof are provided in the current invention. In addition, fusion proteins, antigenic peptides, and antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and cells comprising the expression vectors. Methods for producing the polypeptides of the invention and methods for their use are further provided.
Claims29
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising a nucleotide sequence as set forth in SE+Q ID NO:
- 3;
- b.) a nucleic acid molecule comprising a nucleotide sequence having at least 90% sequence identity to SEQ ID NO:
- 3, wherein said nucleic acid molecule encodes a polypeptide having oxalate degrading activity;
- c) a nucleic acid molecule comprising at least 50 contiguous nucleotides of SEQ ID NO:
- 3, wherein said nucleic acid molecule encodes a polypeptide having oxalate degrading activity;
- d) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:4;
- and,
- e) a nucleic acid molecule that hybridizes under stringent conditions to any of a)-d), wherein said nucleic acid molecule encodes a polypeptide having oxalate degrading activity.
A bacteria comprising
- (a) a first nucleic acid molecule selected from the group consisting of:
- i) a nucleic acid molecule comprising a nucleotide sequence as set forth in SEQ ID NO:3;
- ii) a nucleic acid molecule comprising a nucleotide sequence having at least 90% sequence identity to SEQ ID NO:3, wherein said nucleotide sequence encodes a polypeptide having oxalate degrading activity;
- iii) a nucleic acid molecule comprising a fragment having 50 contiguous nucleotides of SEQ ID NO:3, wherein said nucleotide sequence encodes a polypeptide having oxalate degrading activity;
- iv) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:4;
- and,
- (b) a second nucleic acid molecule selected from the group consisting of:
- i) a nucleic acid molecule comprising a nucleotide sequence as set forth in SEQ ID NO:1;
- ii) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having oxalate degrading activity;
- iii) a nucleic acid molecule comprising a fragment having at least 50 contiguous nucleotides of SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having oxalate degrading activity;
- iv) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:2;
- and,
- where at least one of said first or said second nucleic acid molecule is heterologous to the bacteria and said first and said nucleic acid molecules are operably linked to a promoter active in said bacteria.
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:
- 4;
- b) a polypeptide comprising a fragment having at least 50 contiguous amino acids of SEQ ID NO:
- 4, wherein said polypeptide has oxalate degrading activity;
- and,
- c) a polypeptide having at least 90% sequence identity to an amino acid sequence as set forth in SEQ ID NO:
- 4, wherein said polypeptide has oxalate degrading activity.
The method of 19, wherein said cell further comprises a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:1, wherein said nucleotide sequence encodes a polypeptide having oxalate degrading activity.
A method for detecting the presence of a polypeptide in a sample comprising contacting the sample with a compound that selectively binds to a polypeptide and determining whether the compound binds to the polypeptide in the sample;
- wherein said polypeptide is selected from the group consisting of:
- a) a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:
- 4;
- b) a polypeptide comprising a fragment having at least 50 contiguous amino acids of SEQ ID NO:4, wherein said polypeptide has oxalate degrading activity;
- and,
- c) a polypeptide having at least 90% sequence identity to an amino acid sequence as set forth in SEQ ID NO:
- 4, wherein said polypeptide has oxalate degrading activity.
Dependencies are inferred from the claim text.