Optical in vivo imaging contrast agents and methods of use
AssigneeInvitrogen Corporation
InventorsJohn Mauro, Julie Nyhus, Thomas Steinberg, Yu-Zhong Zhang
Life of this patent
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Abstract
Provided is an optical in vivo contrast agent comprising a fluorescent polymeric microsphere, wherein the microsphere is impregnated with a dye having an excitation and emission spectrum compatible with in vivo imaging, and wherein the microsphere is coated with a block copolymer.
Claims31
A method for imaging a site of disease or injury within the vasculature of a subject wherein the method comprises:
- administering to the subject a contrast agent comprising fluorescent microspheres labeled with a dye having an excitation and emission spectrum compatible with in vivo imaging and wherein the microspheres are coated with a surfactant;
- incubating said subject for a sufficient amount of time for the contrast agents to circulate to the disease or injury sites;
- illuminating the contrast agents in the subject with an appropriate wavelength to form an illuminated subject;
- and
- observing the illuminated subject whereby the site of disease or injury is imaged.
A contrast agent for in-vivo imaging of disease or injury sites in a subject comprising a fluorescent microsphere, wherein the microsphere is labeled with a dye having an excitation and emission spectrum compatible with in vivo imaging and wherein the microspheres are coated with a surfactant.
A kit for in-vivo imaging of disease or injury sites in a subject comprising:
- a contrast agent comprising a fluorescent microsphere, wherein the microsphere is labeled with a dye having an excitation and emission spectrum compatible with in vivo imaging and wherein the microspheres are coated with a surfactant;
- packaging;
- and
- instructions for imaging the disease or injury site with the contrast agent.
Dependencies are inferred from the claim text.