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US 2009/0155164 A1Published application
Human c-fms antigen binding proteins
AssigneeAmgen Inc.
InventorsKenneth Allan Brasel, James F. Smothers, Douglas Pat Cerretti, Christopher Mehlin, Jilin Sun, Stephen Foster
Life of this patent
Estimating the term…
Abstract
Antigen binding proteins that bind to human c-fms protein are provided. Nucleic acids encoding the antigen binding protein, vectors, and cells encoding the same are also provided. The antigen binding proteins can inhibit binding of c-fms to CSF-1, reduce monocyte migration into tumors, and reduce the accumulation of tumor-associated macrophages.
Claims67
An isolated human antigen binding protein that specifically binds to a human c-fms polypeptide.
An isolated antigen binding protein that binds human c-fms comprising:
- A) one or more heavy chain complementary determining regions (CDRHs) selected from the group consisting of:
- (i) a CDRH1 selected from the group consisting of SEQ ID NO:136-147;
- (ii) a CDRH2 selected from the group consisting of SEQ ID NO:148-164;
- (iii) a CDRH3 selected from the group consisting of SEQ ID NO:165-190;
- and
- (iv) a CDRH of (i), (ii) and (iii) that contains one or more amino acid substitutions, deletions or insertions of no more than 4 amino acids;
- B) one or more light chain complementary determining regions (CDRLs) selected from the group consisting of
- (i) a CDRL1 selected from the group consisting of SEQ ID NO:191-210;
- (ii) a CDRL2 selected from the group consisting of SEQ ID NO:211-224;
- (iii) a CDRL3 selected from the group consisting of SEQ ID NO:225-246;
- and
- (iv) a CDRL of (i), (ii) and (iii) that contains one or more amino acid substitutions, deletions or insertions of no more than 4 amino acids;
- or
- C) one or more heavy chain CDRHs of A) and one or more light chain CDRLs of B).
11 more elements
An isolated antigen binding protein that binds human c-fms, wherein said antigen binding protein comprises:
- A) one or more heavy chain CDRs (CDRHs) selected from the group consisting of:
- (i) a CDRH1 with at least 80% sequence identity to SEQ ID NO:136-147;
- (ii) a CDRH2 with at least 80% sequence identity to SEQ ID NO:148-164;
- and
- (iii) a CDRH3 with at least 80% sequence identity to SEQ ID NO:165-190;
- B) one or more light chain CDRs (CDRLs) selected from the group consisting of
- (i) a CDRL1 with at least 80% sequence identity to SEQ ID NO:191-210;
- (ii) a CDRL2 with at least 80% sequence identity to SEQ ID NO:211-224;
- and
- (iii) a CDRL3 with at least 80% sequence identity to SEQ ID NO:225-246;
- or
- C) one or more heavy chain CDRHs of A) and one or more light chain CDRLs of B).
9 more elements
An isolated antigen binding protein that binds human c-fms, wherein said antigen binding protein comprises:
- (A) a heavy chain complementary determining region (CDRH) selected from the group consisting of
- (1) a CDRH3 selected from the group consisting of SEQ ID NOs:165-190,
- (2) a CDRH3 that differs in amino acid sequence from the CDRH3 of (i) by an amino acid addition, deletion or substitution of not more than two amino acids;
- and
- (3) a CDRH3 amino acid sequence selected from the group consisting of
- (a) X1X2X3X4X5FGEX6X7X8X9FDY (SEQ ID NO:309), wherein
- X1 is selected from the group consisting of E and D,
- X2 is selected from the group consisting of S and Q,
- X3 is selected from the group consisting of G and no amino acid,
- X4 is selected from the group consisting of L and no amino acid,
- X5 is selected from the group consisting of W and G,
- X6 is selected from the group consisting of V and L,
- X7 is selected from the group consisting of E and no amino acid,
- X8 is selected from the group consisting of G and no amino acid, and
- X9 is selected from the group consisting of F and L;
- (SEQ ID NO:315)
- (b)
- X1X2X3X4X5X6X7X8X9X10X11X12X13YYGX14DV,
- wherein
- X1 is selected from the group consisting of E, D and G,
- X2 is selected from the group consisting of Y, L and no amino acid,
- X3 is selected from the group consisting of Y, R, G and no amino acid,
- X4 is selected from the group consisting of H, G, S and no amino acid,
- X5 is selected from the group consisting of I, A, L and no amino acid,
- X6 is selected from the group consisting of L, V, T, P and no amino acid,
- X7 is selected from the group consisting of T, V, Y, G, W and no amino acid,
- X8 is selected from the group consisting of G, V, S and T,
- X9 is selected from the group consisting of S, T, D, N and G,
- X10 is selected from the group consisting of G, F, P, and Y,
- X11 is selected from the group consisting of G, Y and N,
- X12 is selected from the group consisting of V and Y,
- X13 is selected from the group consisting of W, S and Y, and
- X14 is selected from the group consisting of M, T and V;
- and
- (c) SSX1X2X3YX4MDV (SEQ ID NO:321), wherein
- X1 is selected from the group consisting of G, S and W,
- X2 is selected from the group consisting of N, D and S,
- X3 is selected from the group consisting of Y and F, and
- X4 is selected from the group consisting of D and G;
- or
- (B) a light chain complementary determining region (CDRL) selected from the group consisting of
- (1) a CDRL3 selected from the group consisting of SEQ ID NOs:225-246,
- (2) a CDRL3 that differs in amino acid sequence from the CDRL3 of (i) by an amino acid addition, deletion or substitution of not more than two amino acids;
- and
- (3) a CDRL3 amino acid sequence selected from the group consisting of
- (a) QQYYX1X2PX3T (SEQ ID NO:312), wherein
- X1 is selected from the group consisting of S and T,
- X2 is selected from the group consisting of D and T, and
- X3 is selected from the group consisting of F and P;
- (b) QQYDX1LX2T (SEQ ID NO:318), wherein
- X1 is selected from the group consisting of N and D, and
- X2 is selected from the group consisting of L and I;
- and
- (c) QX1YDX2X3PX4T (SEQ ID NO:324), wherein
- X1 is selected from the group consisting of Q and R,
- X2 is selected from the group consisting of N and D,
- X3 is selected from the group consisting of L and F, and
- X4 is selected from the group consisting of F, L and I.
55 more elements
An antigen binding protein that comprises a CDRH1, a CDRH2, a CDRH3, a CDRL1, a CDRL2, and a CDRL3 each with a sequence as specified in a line of Table 4C.
Dependencies are inferred from the claim text.