Complex formation method and separation method
AssigneeWako Pure Chemicals Industries, Ltd.
InventorsTomohisa Kawabata, Shinji Satomura, Henry Garrett Wada
Life of this patent
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Abstract
An object of the present invention is to provide a method for forming a complex between an analyte or an analogue thereof, and a substance formable the complex with said analyte or said analogue thereof (the complex forming substance), in a short time and in high reaction efficiency, and a method for separating a complex formed, and a complex forming substance not involved in formation of said complex or an analogue not involved in formation of said complex rapidly, simply and in high accuracy, along with a method for measuring an analyte in a sample in high sensitivity.
Claims18
A method for forming a complex comprising the following steps:
- (1) a step of arranging (a) a solution containing an analyte or an analogue thereof and (b) a solution containing not less than one kind of a substance capable of forming a complex with said analyte or said analogue thereof (the complex forming substance), in a capillary, so that by applying a voltage to said capillary the complex between said analyte or said analogue thereof and the complex forming substance are formed without mixing these solutions in advance;
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- (2) a step of contacting said analyte or said analogue thereof with the complex forming substance while concentrating said analyte or said analogue thereof and/or at least one kind of the complex forming substances by applying a voltage to said capillary before uniformly mixing these solutions to form the complex between said analyte or said analogue thereof and the complex forming substance.
A method for separating a complex comprising the following steps:
- (1) a step of arranging (a) a solution containing an analyte or an analogue thereof and (b) a solution containing not less than one kind of a substance capable of forming a complex with said analyte or said analogue thereof (the complex forming substance), in a capillary, so that by applying a voltage to said capillary the complex between said analyte or said analogue thereof and the complex forming substance are formed without mixing these solutions in advance;
- (2) a step of contacting said analyte or said analogue thereof with the complex forming substance while concentrating said analyte or said analogue thereof and/or at least one kind of the complex forming substances by applying a voltage to said capillary before uniformly mixing these solutions to form the complex between said analyte or said analogue thereof and the complex forming substance;
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- (3) a step of separating said complex, and the complex forming substance not involved in the formation of said complex or the analogue not involved in the formation of said complex by further electrical movement.
A method for measuring an analyte comprising the following steps:
- (1) a step of arranging (a) a solution containing an analyte or an analogue thereof and (b) a solution containing not less than one kind of a substance formable the complex with said analyte or said analogue thereof (the complex forming substance), in a capillary, so that by applying a voltage to said capillary the complex between said analyte or said analogue thereof and the complex forming substance are formed without mixing these solutions in advance;
- (2) a step of contacting said analyte or said analogue thereof with the complex forming substance while concentrating said analyte or said analogue thereof and/or at least one kind of the complex forming substances by applying a voltage to said capillary before uniformly mixing these solutions to form the complex between said analyte or said analogue thereof and the complex forming substance;
- (3) a step of separating said complex, and the complex forming substance not involved in the formation of said complex or the analogue not involved in the formation of said complex by further electrical movement;
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- (4) a step of measuring the amount of thus separated complex, or the amount of the complex forming substance or the analogue not involved in the formation of said complex to determine the amount of said analyte based on the result.
Dependencies are inferred from the claim text.