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US 2009/0148950 A1Published application
Production of packaged dna sequences
AssigneeWisconsin Alumni Research Foundation
InventorsPaul G. Ahlquist, Dohun Pyeon, Paul F. Lambert
Life of this patent
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Abstract
A method of producing a packaged DNA sequence is disclosed. In one embodiment, the method comprises the steps of: (a) selecting a DNA sequence to be packaged and a papillomaviral capsid sequence, wherein the DNA sequence to be packaged is between 7 Kb-8.5 Kb, (b) co-transfecting the products of step (a) into transfectable cells, wherein the DNA sequence is packaged, and (c) purifying packaged particles.
Claims19
A method of producing a packaged DNA sequence comprising the steps of:
- (a) selecting a DNA sequence to be packaged and papillomoviral capsid sequences L1 and L2 wherein the DNA sequence is a full-length or near full-length papillomaviral genomic DNA sequence of at least 7 Kb,
- (b) co-transfecting the products of step (a) into transfectable cells, wherein the DNA sequence is packaged, and
- (c) purifying packaged particles.
(canceled)
A method of producing a packaged DNA sequence comprising the steps of:
- (a) selecting a DNA sequence to be packaged and papillomaviral capsid sequences L1 and L2, wherein the DNA sequence to be packaged is between 7 Kb-8.5 Kb,
- (b) co-transfecting the products of step (a) into transfectable cells, wherein the DNA sequence is packaged, and
- (c) purifying packaged particles wherein the yield of particles is at least 50 protected DNA copies per cell.
(canceled)
Dependencies are inferred from the claim text.