Method for producing amino acids using bacterium of the enterobacteriaceae family
AssigneeAjinomoto Co., Inc.
InventorsRustem Saidovich Shakulov, Elena Vitalievna Klyachko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh
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Abstract
A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
Claims11
An L-amino acid producing bacterium of the Enterobacteriaceae family comprising a DNA comprising:
- A) a gene encoding a bacterial enzyme which influences L-amino acid biosynthesis, and
- B) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO:
- 2, or a variant thereof, wherein said fragment is attached to said gene at the 3′ end of said gene.
Dependencies are inferred from the claim text.