Method for preparing sample for analysis of dioxins

An apparatus for preparing a sample for analysis of dioxins from an aliphatic hydrocarbon solvent solution of dioxins, comprising:

• a first column packed with a silica gel layer,
• a second column packed with an alumina layer and a carbon material layer in a layered state,
• a supply passage for supplying the aliphatic hydrocarbon solvent solution to the first column,
• a first solvent supply section for supplying an aliphatic hydrocarbon solvent to the first column,
• a column communicating passage for supplying the aliphatic hydrocarbon solvent from the first column to the second column from the alumina layer side,
• a second solvent supply section for supplying one extracting solvent selected from the group consisting of toluene, a mixed solvent of toluene and an aliphatic hydrocarbon solvent, and a hydrophilic solvent capable of dissolving the dioxins to the second column from the carbon material layer side, and
• a discharge passage for discharging the extracting solvent passed through the second column.

A column for preparing a sample for analysis of dioxins, comprising:

• a cylindrical container having an opening portion at both ends;
• a carbon material layer packed in the container;
• and
• an alumina layer packed adjacent to the carbon material layer in the container.

A method for analyzing dioxins, which comprises the steps of:

• supplying an aliphatic hydrocarbon solvent solution of dioxins to an alumina layer,
• supplying an aliphatic hydrocarbon solvent to the alumina layer, to which the aliphatic hydrocarbon solvent solution has been supplied, thereby passing the aliphatic hydrocarbon solvent through the alumina layer,
• supplying the aliphatic hydrocarbon solvent passed through the alumina layer to a carbon material layer, thereby passing the aliphatic hydrocarbon solvent through the carbon material layer,
• supplying one extracting solvent selected from the group consisting of toluene and a mixed solvent of toluene and an aliphatic hydrocarbon solvent to the carbon material layer and the alumina layer after passing the aliphatic hydrocarbon solvent through the carbon material layer, in the direction opposed to the passing direction of the aliphatic hydrocarbon solvent, and
• injecting a solution of the extracting solvent obtained by passing through the carbon material layer and the alumina layer in this order to a gas chromatograph-mass spectrometer as it is, thereby analyzing the dioxins.

A method for analyzing dioxins, which comprises the steps of:

• supplying an aliphatic hydrocarbon solvent solution of dioxins to an alumina layer,
• supplying an aliphatic hydrocarbon solvent to the alumina layer, to which the aliphatic hydrocarbon solvent solution has been supplied, thereby passing the aliphatic hydrocarbon solvent through the alumina layer,
• supplying the aliphatic hydrocarbon solvent passed through the alumina layer to a carbon material layer, thereby passing the aliphatic hydrocarbon solvent through the carbon material layer,
• supplying a hydrophilic solvent capable of dissolving the dioxins to the carbon material layer and the alumina layer after passing the aliphatic hydrocarbon solvent through the carbon material layer in the direction opposed to the passing direction of the aliphatic hydrocarbon solvent, thereby securing a solution of the hydrophilic solvent obtained by passing through the carbon material layer and the alumina layer in this order, and
• analyzing the dioxins contained in the solution of the hydrophilic solvent using a bioassay method.