Porcine umami taste receptors and uses therefor
AssigneesThe Regents of the University of California Office of Technology Transfer, Lucta
InventorsKirk Klasing, Roseline Holt, Eugeni Roura
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Abstract
The present invention provides nucleic acids encoding porcine taste receptors, polypeptides encoded by the nucleic acids, and methods of using the nucleic acids and polypeptides to identify compounds that enhance umami taste.
Claims27
An isolated umami taste receptor, comprising a first taste receptor polypeptide selected from the group consisting of:
- a pT1R1 polypeptide encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence of SEQ ID NO:1 or a complement thereof;
- and
- a pT1R3 polypeptide encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence of SEQ ID NO:2 or a complement thereof.
A method of identifying a compound that modulates taste signal transduction in taste cells, the method comprising the steps of
- (i) contacting the compound with a taste receptor comprising a first taste receptor polypeptide selected from the group consisting of:
- a pT1R1 polypeptide encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence of SEQ ID NO:1 or a complement thereof;
- and
- a pT1R3 polypeptide encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence of SEQ ID NO:2 or a complement thereof;
- and
- (ii) determining a physical or functional effect of the compound upon the receptor, thereby identifying a compound that modulates taste signal transduction.
An isolated polynucleotide comprising a nucleic acid sequence sharing at least 95% sequence identity to SEQ ID NO:1 or SEQ ID NO:2.
An isolated nucleic acid comprising the sequence set forth in SEQ ID NO:3, 4, 5, 6, 7, 8, 9, 10, 11, or 12.
An isolated polypeptide comprising an amino acid sequence sharing at least 95% sequence identity to SEQ ID NO:13 or SEQ ID NO:14.
Dependencies are inferred from the claim text.