Vaccine for nontypeable haemophilus influenzae infection
AssigneeThe Research Foundation of State University of New York
InventorsThomas Hiltke, Timothy F. Murphy, Sanjay Sethi
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Abstract
This invention provides a method for identifying strains of nontypeable Haemophilus influenzae that can be used as immunogenic compositions having specificity against a plurality of Haemophilus influenzae strains. The invention also provides compositions comprising whole cell bacteria or outer membrane protein P2 obtained from the strains for use as vaccines.
Claims10
An immunogenic composition comprising whole cell bacteria from at least five strains of nontypeable Haemophilus influenzae (NTHI), wherein each of the strains can elicit an immune response against at least one NTHI strain not included in the immunogenic composition.
A method for designing immunogenic compositions having cross reactivity against a plurality of nontypeable Haemophilus influenzae (NTHI) strains comprising the steps of:
- a) obtaining amino acid sequences of NTHI outer membrane protein 2 (OMP P2) proteins from a plurality of NTHI strains;
- b) comparing the sequences and constructing a relatedness tree;
- c) grouping the strains based on degrees of relatedness of the OMP P2 amino acid sequences;
- d) from the groups of step c), selecting one or more representative strains having high degrees of OMP P2 amino acid sequence relatedness to other strains in the group from which the representative is selected;
- e) testing the selected representative strains to identify representative strains with high degrees of cross-reactivity to a plurality of NTHI strains;
- and
- f) preparing an immunogenic composition comprising whole cell bacteria from the representative strains identified in step e) or OMP P2 proteins derived from the representative strains identified in step e).
Dependencies are inferred from the claim text.