Methionyl tRNA Synthetase Polynucleotides
AssigneeReplidyne, Inc.
InventorElizabeth Jane Lawlor
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Abstract
The invention provides metS polypeptides and DNA (RNA) encoding metS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing metS polypeptides to screen for antibacterial compounds.
Claims24
1-13. (canceled)
An antagonist which inhibits the activity or expression of the polypeptide represented by SEQ ID NO:
- 2 or 4 wherein the polypeptide exhibits methionyl tRNA synthetase activity.
-17. (canceled)
A method for identifying compounds which interact with and inhibit an activity of the polypeptide represented by SEQ ID NO:
- 2 or 4, the method comprising:
- contacting a composition comprising the polypeptide with the compound under conditions to permit interaction between the compound and the polypeptide to assess the interaction of the compound, such interaction being associated with a component capable of providing a detectable signal in response to the interaction of the polypeptide with the compound;
- and
- determining whether the compound interacts with and inhibits an activity of the polypeptide by detecting the presence or absence of a signal generated from the interaction of the compound with the polypeptide.
19-20. (canceled)
A method for identifying compounds which inhibit an activity of the polypeptide represented by SEQ ID NO:
- 2 or 4, the method comprising:
- contacting a cell expressing on the surface thereof a substrate for binding the polypeptide, said substrate being associated with a second component capable of providing a detectable signal in response to the binding of a compound to the substrate, with a compound to be screened under conditions to permit binding to the substrate;
- and
- determining whether the compound binds to the substrate by detecting the presence or absence of a signal generated from the interaction of the compound with the substrate.
A method of screening drugs to identify those which interfere with the interaction of the methionyl tRNA synthetase represented by SEQ ID NO:
- 2 or 4, wherein the method comprises measuring enzyme activity by the full aminoacylation reaction or the partial PPi/ATP exchange reaction.
Dependencies are inferred from the claim text.