Nucleic acid derivatives and methods of use
AssigneeBioDynamics Laboratory, Inc
InventorsToshiyuki Kato, Hitoshi Nagahora, Masaaki Onda, Shingo Tanaka
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Abstract
The objective of the invention is to provide nucleic acid derivatives with designated compounds introduced specifically into the base of nucleic acids. A DNA or RNA, or a nucleic acid molecular weight marker, wherein a designated compound (R), which is selected from any one of dyes, fluorescent dyes, RI labeled substances, and compounds capable of linking specifically to other compounds, is introduced at the N−3 position of a thymine base or uracil base in which (R′) at Position 1 is other than hydrogen atom.
Claims18
A nucleic acid derivative wherein a designated compound (R) is introduced at the N−3 position of a thymine base or uracil base represented by the following structural formula (I)
- in which (R′) at Position 1 is other than hydrogen atom, via a vinyl sulfone group represented by the following structural formula (II)
- or a sulfatoethyl sulfone group represented by the following structural formula (III).
A nucleic acid molecular weight marker, said marker being a DNA or RNA, wherein a designated compound (R) is linked to the N−3 position of a thymine base or uracil base represented by the following structural formula (IV)
- in which (R′) at Position 1 is other than hydrogen atom, via a vinyl sulfone group represented by the following structural formula (V)
- or a sulfatoethyl sulfone group represented by the following structural formula (VI).
A process for the preparation of a nucleic acid derivative into which a designated compound (R) is introduced, said process comprising
- a step of having a given nucleic acid made available;
- and
- a step of linking the designated compound (R) to the N−3 position of a thymine base or uracil base of said nucleic acid via a vinyl sulfone group represented by the following structural formula (VII)
- or a sulfatoethyl sulfone group represented by the following structural formula (VIII).
Dependencies are inferred from the claim text.