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US 2009/0081788 A1Published application
Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
AssigneesInstitut Pasteur, Universite Pierre et Marie Curie
InventorsBernard Dujon, Andre Choulika, Arnaud Perrin, Jean-Francois Nicolas
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Abstract
An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
Claims2
A method of inducing at least one site-directed double-strand break in DNA of a cell, said method comprising
- (a) providing cells containing double-stranded DNA, wherein said DNA comprises at least one I-SceI restriction site;
- (b) transfecting said cells with at least a plasmid comprising DNA encoding the I-SceI meganuclease;
- and
- (c) selecting cells in which at least one double-strand break has been induced.
1 more element
2-22. (canceled)
Dependencies are inferred from the claim text.