Vector expressing n-deacetylase/n-sulfotransferase 2
AssigneeRiken
InventorsJun-ichi Aikawa, Masafumi Tsujimoto, Takuji Kaneko
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Abstract
An object of the present invention is to provide an expression vector that allows for stable production of N-deacetylase/N-sulfotransferase 2 in large amounts and a process for production of N-deacetylase/N-sulfotransferase 2 using the same. The present invention provides a recombinant baculovirus expression vector obtained by incorporating into baculovirus DNA, a DNA fragment having lobster L21 DNA, DNA encoding gp67 signal peptide and DNA encoding the 79th to 883rd amino acids of human N-deacetylase/N-sulfotransferase 2 in this order in the 5′ to 3′ direction.
Claims14
A human N-deacetylase/N-sulfotransferase 2 of the following (a) or (b), wherein the N-terminal sequence of the transmembrane domain has been removed:
- (a) a protein comprised of an amino acid sequence at the 79th to 883rd positions of SEQ ID NO:
- 2;
- or
- (b) a protein comprised of an amino acid sequence of the protein (a) with the deletion, substitution, insertion, or transposition of one or several amino acids, and having N-deacetylase activity and N-sulfotransferase activity.
A recombinant baculovirus expression vector obtained by incorporating into baculovirus DNA, a DNA fragment having at least DNA encoding a signal peptide capable of functioning in a baculovirus expression vector and DNA encoding the 79th to 883rd amino acids of human N-deacetylase/N-sulfotransferase 2 or DNA derived from the DNA with deletion, insertion, or substitution of one or several nucleotides and encoding a protein exhibiting enzyme activity equal to that of human N-deacetylase/N-sulfotransferase 2 in this order in the 5′ to 3′ direction.
Dependencies are inferred from the claim text.