Kappa-carrageenase and kappa-carrageenase-containing compositions
OwnerHugh C. McDonald, Brian Schmidtinventors
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Abstract
The present invention provides cleaning compositions comprising at least one carrageenase enzyme, methods for producing carrageenase enzymes in host cells, host cells comprising recombinant polynucleotides encoding at least one carrageenase, and recombinant polynucleotides encoding carrageenase.
Claims30
An isolated recombinant polynucleotide comprising a sequence encoding a kappa-carrageenase polypeptide, said sequence being operably linked to a polynucleotide encoding a secretory signal peptide.
A Bacillus host cell comprising:
- a recombinant polynucleotide encoding a fusion protein comprising:
- a) a signal sequence;
- and
- b) carrageenase protein;
- wherein said host cell secretes said kappa-carrageenase protein from said cell and wherein said host cell has at least one inactivated protease gene.
The method of claim 21, further comprising the step of harvesting said produced carrageenase.
A cleaning composition comprising an effective amount of an isolated kappa-carrageenase comprising an amino acid sequence that is at least about 70% identical to the kappa-carrageenase of SEQ ID NO:4.
Dependencies are inferred from the claim text.