Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
AssigneesGen-Probe Incorporated, Biomerieux
InventorsYeasing Y. Yang, Steven T. Brentano, Odile Babola, Nathalie Tran, Guy Vernet
Life of this patent
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Abstract
Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences are disclosed.
Claims21
A mixture of nucleic acid oligomers for specifically amplifying a nucleotide sequence of HIV-1 in a pol gene referred to as a first reverse transcriptase or RT1 sequence, comprising at least one oligomer selected from the group consisting of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:52, and SEQ ID NO:53, and a second oligomer that permits amplification of about 532 nucleotides of HIV-1 pol sequence.
A method of detecting HIV-1 in a biological sample, comprising the steps of:
- providing a biological sample containing HIV-1 nucleic acid;
- mixing the sample with two or more amplification oligomers that specifically amplify at least one HIV-1 target sequence contained within a pot gene referred to as a first reverse transcriptase or RT1 sequence under conditions that allow amplification of nucleic acid, wherein the amplification oligomers contain a first oligomer selected from the group consisting of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:52, and SEQ ID NO:53, and a second oligomer that permits amplification of the RT1 sequence to amplify an HIV-1 pol sequence;
- amplifying the target sequence in the RT1 sequence to produce an amplified RT1 nucleic acid product about 532 nucleotides;
- and
- detecting the presence of the amplified RT1 nucleic acid product.
Dependencies are inferred from the claim text.