Method for cloning and expression of stui restriction endonuclease and stui methylase in e. coli
AssigneeNew England Biolabs, Inc.
InventorsZhenyu Zhu, Shuang-Yong Xu
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Abstract
The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.
Claims7
An isolated DNA encoding a restriction endonuclease capable of recognizing AGGCCT, comprising:
- a nucleotide sequence having at least 65% sequence identity with SEQ ID NO:1 wherein the restriction endonuclease has at least 50% sequence identity with SEQ ID NO:2.
A DNA segment encoding a methylase capable of methylating one or more nucleotides in a nucleotide sequence recognized by the StuI restriction endonuclease (StuIR), the DNA segment having at least 65% sequence identity with SEQ ID NO:3 wherein the methylase has at least 50% sequence identity with SEQ ID NO:4.
Dependencies are inferred from the claim text.