Holographic microscopy of holographically trapped three-dimensional structures
AssigneeNew York University
InventorsSang-Hyuk LEE, David G. Grier
Life of this patent
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Abstract
A method and system for performing three-dimensional holographic microscopy of an optically trapped structure. The method and system use an inverted optical microscope, a laser source which generates a trapping laser beam wherein the laser beam is focused by an objective lens into a plurality of optical traps. The method and system also use a collimated laser at an imaging wavelength to illuminate the structure created by the optical traps. Imaging light scattered by the optically tapped structure forms holograms that are imaged by a video camera and analyzed by optical formalisms to determine light field to reconstruct 3-D images for analysis and evaluation.
Claims20
A method of performing 3-D holographic microscopy of an optically trapped structure, comprising the steps of:
- providing an optical microscope;
- generating a laser beam having an associated imaging wavelength input to the inverted optical microscope;
- generating a plurality of optical traps with wave fronts of a trapping laser beam having a phase only hologram encoding a desired optical trapping pattern and the trapping laser beam having an associated trapping wavelength;
- and
- providing an objective lens for the inverted optical microscope, the objective lens both projecting the plurality of optical traps and collecting in-line holographic images of the trapped structure;
- and
- providing images of the trapped structure to a CCD camera for three-dimensional holographic microscopy of the trapped structure.
A system for performing three-dimensional holographic microscopy of an optically trapped structure, comprising:
- an inverted optical microscope;
- a laser source for producing a trapping laser beam;
- a spatial light modulator for providing a phase-only hologram for imprintation on the trapping laser beam;
- a laser source for producing an imaging laser beam an objective lens associated with the inverted optical microscope;
- a CCD camera for detecting laser light arising from imaging of the trapped structure and the CCD camera outputting image data;
- a computer for analyzing the image data using computer software executed by the computer.
Dependencies are inferred from the claim text.