Glutamate receptor associated genes and proteins for enhancing nitrogen utilization efficiency in crop plants
AssigneesPioneer Hi-Bred International, Inc., The George Washington University
InventorsHOWARD P. HERSHEY, MARY J. FRANK, CARL R. SIMMONS, FRANK J. TURANO
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Abstract
The invention provides isolated glutamate receptor associated nucleic acids and their encoded proteins for modulating nitrogen utilization efficiency in plants. The invention includes methods and compositions relating to altering nitrogen utilization and/or uptake in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
Claims31
An isolated polynucleotide selected from the group consisting of:
- a. a polynucleotide having at least 70% sequence identity, as determined by the GAP algorithm under default parameters, to the full length sequence of a polynucleotide selected from the group consisting of SEQ ID NOS:1, 3, 5, 7, and 9;
- wherein the polynucleotide encodes a polypeptide that functions as a modifier of nitrogen utilization efficiency;
- b. a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NOS:2, 4, 6, 8, and 10;
- c. a polynucleotide selected from the group consisting of SEQ ID NOS:1, 3, 5, 7, and 9;
- and
- d. A polynucleotide which is complementary to the polynucleotide of (a), (b), or (c).
A method of decreasing the glutamate receptor associated polypeptide activity in a plant cell, comprising:
- a. providing a nucleotide sequence comprising at least 15 consecutive nucleotides of the complement of SEQ ID NO:
- 1, 3, 5, 7, or 9;
- b. providing a plant cell comprising an mRNA having the sequence set forth in SEQ ID NO:
- 1, 3, 5, 7, or 9;
- and
- c. introducing the nucleotide sequence of step (a) into the plant cell of step (b), wherein the nucleotide sequence inhibits expression of the mRNA in the plant cell.
Dependencies are inferred from the claim text.