DWF12 and Mutants Thereof

An isolated dwf12 mutant polypeptide with at least about 70% sequence identity to the polypeptide depicted at positions 38-326 or 1-380 of FIG. 3 or a polypeptide designated in Table 1, wherein said polypeptide comprises a mutation of at least one non-conservative substitution, addition or deletion of an amino acid in a region of the polypeptide corresponding to a region depicted in FIG. 3, wherein said region is one or more of (a) a casein kinase II phosphorylation domain spanning positions 104-106 of FIG. 3;

• (b) a casein kinase II phosphorylation domain spanning positions 220-223 of FIG. 3;
• (c) a casein kinase II phosphorylation domain spanning positions 261-264 of FIG. 3;
• (d) a casein kinase II phosphorylation domain spanning positions 314-317 of FIG. 3;
• (e) a casein kinase II phosphorylation domain spanning positions 353-356 (f) a protein kinase C phosphorylation domain spanning positions 104-106 of FIG. 3;
• (g) a protein kinase C phosphorylation domain spanning positions 187-189 of FIG. 3;
• (h) a protein kinase C phosphorylation domain spanning positions 310-312;
• (i) the active site lysine residue found at position 69 of FIG. 3;
• (j) a tyrosine phosphorylation domain spanning positions 70-77 of FIG. 3;
• (k) a protein kinase ATP-binding domain spanning positions 46-69 of FIG. 3;
• (l) a eukaryotic protein kinase ATP-binding domain spanning positions 157-172 of FIG. 3;
• (m) a eukaryotic protein kinase substrate-binding domain spanning positions 218-228 of FIG. 3;
• (n) a eukaryotic protein kinase signature sequence spanning positions 316-327 of FIG. 3;
• (o) a glycosylation domain spanning positions 197-200 of FIG. 3;
• (p) a glycosylation domain spanning positions 376-379 of FIG. 3;
• (q) the sequence CDFGSAK spanning positions 183-189 of FIG. 3;
• or (r) the sequence SYICSR spanning positions 199-204 of FIG. 3.