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US 2009/0023902 A1Published application
Method for the extraction of intracellular proteins from a fermentation broth
AssigneeDow Global Technologies LLC
InventorsTimothy C. Frank, Felipe A. Donate, Jefry E. Shields, Kai Li, Jeffrey R. Allen
Life of this patent
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Abstract
A method for extracting an intracellular peptide, protein or other polypeptide from a whole fermentation broth using a water miscible alcohol, or a water miscible or partially water miscible glycol ether.
Claims21
A method for extracting an intracellular protein from a fermentation broth comprising the steps of:
- (a) intermixing a sufficient quantity of a water miscible alcohol or a glycol ether with an aqueous fermentation broth at a temperature at which a single phase comprising a protein, the water miscible alcohol or the glycol ether, and water is formed;
- (b) separating the phase comprising the protein, the water miscible alcohol or the glycol ether, and water formed in step (a) from solid biomass impurities;
- and, optionally,
- (c) recovering the protein from the phase obtained in step (b) by any conventional protein recovery method.
1 more element
(canceled)
A method for extracting an intracellular protein from a fermentation broth comprising the steps of:
- (a) intermixing a sufficient quantity of a partially water miscible glycol ether with an aqueous fermentation broth at a temperature such that two phases are formed, a first phase comprising a protein, partially water miscible glycol ether, and water;
- and a second phase comprised mainly of partially miscible glycol ether;
- (b) separating the first phase formed in step (a) from the second phase,
- (c) separating the first phase obtained in step (b) from solid biomass impurities;
- and, optionally,
- (d) recovering the protein from the first phase obtained in step (c) by any conventional protein recovery method.
3 more elements
A method for extracting an intracellular protein from a fermentation broth comprising the steps of:
- (a) intermixing a sufficient quantity of a partially water miscible glycol ether with an aqueous fermentation broth at a temperature such that two phases are formed, a first phase comprised mainly of a partially water miscible glycol ether, and water;
- and a second phase comprising a protein and partially miscible glycol ether;
- (b) separating the second phase formed in step (a) from the first phase,
- (c) separating the second phase obtained in step (b) from solid biomass impurities;
- and, optionally,
- (d) recovering the protein from the second phase obtained in step (c) by any conventional protein recovery method.
3 more elements
Dependencies are inferred from the claim text.