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US 2009/0023670 A1Published application
Regulation of Transgene Expression by RNA Interference
OwnerMagdolna G. Sebestyeninventor
Life of this patent
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Abstract
Expression of transgenes delivered into a host organism cells can be regulated by RNA effector molecules delivered to or present in the host organism cells. Regulation can be mediated by delivery of RNA interference inducing molecules that target the transgene mRNA or by incorporating engineered RNA effector binding sites into the transgene. Temporary or long term regulation of expression can be achieved depending on the nature and dosing of RNA effector.
Claims30
A method regulating transgene expression in vivo comprising:
- delivering an RNA effector molecule to the cell in which the transgene is expressed wherein the RNA effector molecule contains sequence that is complementary to a sequence present in a messenger RNA transcribed from the transgene.
A method for regulating transgene expression in vivo comprising:
- a) forming a transgene expression cassette wherein the expression cassette contains an RNA effector binding site for a cognate RNA effector molecule wherein the RNA effector binding site is present on a messenger RNA transcribed from the transgene expression cassette and
- b) delivering the transgene to a cell in vivo.
A transgene expression cassette comprising:
- a promoter, a coding sequence for a transgene, and one or more ectopic RNA effector binding sites.
Dependencies are inferred from the claim text.