Method of using broad-spectrum delta-endotoxins
AssigneeMonsanto Technology LLC
InventorsThomas Malvar, Komarlingam Sukavaneaswaran Mohan, Sakuntala Sivasupramaniam
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Abstract
Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
Claims21
1-32. (canceled)
A method of controlling insects comprising contacting said insects with an insecticidal Bacillus thuringiensis hybrid protein comprising domains I and II of a first native B. thuringiensis delta-endotoxin Cry1A protein, domain III of a second native B. thuringiensis delta-endotoxin Cry1F protein, and all or a portion of a protoxin segment of a native delta endotoxin Cry1A, Cry1F or combination Cry1F-Cry1A protein.
A method of controlling insects comprising contacting said insects with more than one insecticidal Bacillus thuringiensis hybrid crystal protein each comprising domains I and II of a first native B. thuringiensis delta-endotoxin Cry1A protein, domain III of a second native B. thuringiensis delta-endotoxin Cry1F or Cry1C protein, and all or a portion of a protoxin segment of a native delta endotoxin Cry1A, Cry1F, Cry1C, or combination Cry1F-Cry1A protein.
Dependencies are inferred from the claim text.