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US 2009/0023651 A1Published application
Inhibitors of human plasmin derived from the kunitz domains
AssigneeDyax Copr., a Delaware corporation
InventorsWilliam Markland, Robert C. Ladner
Life of this patent
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Abstract
This invention provides: novel proteins, which are homologous to the first Kunitz domain (K1) of lipoprotein-associated coagulation inhibitor (LACI), and which are capable of inhibiting plasmin; uses of such novel proteins in therapeutic, diagnostic, and clinical methods; and polynucleotides that encode such novel proteins.
Claims2
-11. (canceled)
A method of treating liver cirrhosis or amyloidosis, comprising:
- administering to a subject an effective amount of a polypeptide comprising a non-naturally occurring Kunitz domain that has the formula:
- Met-His-Ser-Phe-Cys-Ala-Phe-Lys-Ala-Xaa10-Xaa11-Gly-Xaa13-Cys-Xaa15-Xaa16-
- Xaa17-Xaa18-Xaa19-Arg-Trp-Xaa22-Xaa23-Asn-Ile-Phe-Thr-Arg-Gln-Cys-Xaa31-Xaa32-Phe-
- Xaa34-Xaa35-Gly-Gly-Cys-Xaa39-Xaa40-Asn-Gln-Xaa43-Arg-Xaa45-Glu-Ser-Leu-Glu-Glu-
- Cys-LysLys Met-Cys-Thr-Arg-Asp,
- wherein Xaa10 is selected from the group consisting of Asp, Glu, and Tyr;
- Xaa11 is selected from the group consisting of Thr, Ala, Ser, Val, and Asp;
- Xaa13 is selected from the group consisting of Pro, Leu, and Ala;
- Xaa15 is selected from the group consisting of Arg and Lys;
- Xaa16 is selected from the group consisting of Ala and Gly;
- Xaa17 is selected from the group consisting of Arg, Lys, and Ser;
- Xaa18 is selected from the group consisting of Phe and Ile;
- Xaa19 is selected from the group consisting of Glu, Asp, Pro, Gly, Ser, and Ile;
- Xaa22 is selected from the group consisting of Tyr and Phe;
- Xaa23 is selected from the group consisting of Tyr and Phe;
- Xaa31 is selected from the group consisting of Asp, Glu, Thr, Val, Gln, and Ala;
- Xaa32 is selected from the group consisting of Thr, Ala, Glu, Pro, and Gln;
- Xaa34 is selected from the group consisting of Val, Ile, Thr, Leu, Phe, Tyr, His, Asp, Ala, and Ser;
- Xaa35 is selected from the group consisting of Tyr and Trp;
- Xaa39 is selected from the group consisting of Glu, Gly, Asp, Arg, Ala, Gln, Leu, Lys, and Met;
- Xaa40 is selected from the group consisting of Gly and Ala;
- Xaa43 is selected from the group consisting of Asn and Gly;
- and
- Xaa45 is selected from the group consisting of Phe and Tyr.
21 more elements
Dependencies are inferred from the claim text.